Upon successful completion of this course students will be able to:
1. Outline the history of major microbiological discoveries and describe
their contributions to world civilization.
2. Describe the steps in the scientific method.
3. Relate basic principles of chemistry and cell biology to microbes.
4. Describe the unique structures and mechanisms of microbial genetics.
5. Categorize microbes taxonomically and evolutionarily.
6. Describe viruses and their relation to cells.
7. Compare various mechanisms of pathogenicity.
8. Describe the function of the immune system and its relation to
9. Perform a variety of microbiological laboratory techniques.
I. Historical development
A. The pre-microbial world
B. Discovery of microbial world, development of the microscope
C. Spontaneous generation and pleiomorphism
D. Koch's postulates
E. Contribution of biochemistry and molecular biology to
F. Contribution of microbiology to world civilization
II. Cell biology
A. Chemistry and biochemistry review
B. Lipids, membranes and cells
C. DNA, RNA, Protein: structure and functions
D. ATP synthesis and cell work
E. The eucaryotic cell - structure and function
F. The procaryotic cell - structure and function
A. Steps of the scientific method
B. Methods of sterilization: heat and filtration
C. Media and their construction and utilization
D. Methods of obtaining pure cultures
E. Staining and microscopy
F. Analysis and manipulation of growth: the standard curve
G. Enrichment culture
H. Fermentation: theory and practice
I. Isolation of mutants and recombinants
J. Virus titration
IV. Microbial genetics
A. Genome and phenotype
B. Mutation, selection, adaptation
1. mobile genetic elements: virus, plasmid
2. enzymes and mechanisms
3. isolation and identification of recombinants
D. The environment and the genome
A. Definitions and historical background
B. Interactions with cells
C. Viral disease
1. vaccination and treatment
2. small pox and polio
3. HIV disease
A. Problems intrinsic to taxonomy
B. Traditional versus modern approaches
C. Taxonomy of selected groups
A. Evolutionary origins.
B. Specific types: nitrogen fixation, cellullose disgestion
C. Impact on model of infectious disease
A. Role of normal flora
B. Mechanisms of pathogenicity
D. Role of the host in disease
1) Non-specific resistance
2) Immune system
3) Factors influencing host resistance
IX. Applied microbiology
A. Modern biotechnology or "genetic" engineering
B. Enrichment culture in biotechnology
C. Traditional enrichment and fermentation biology
Microbiology: An Introduction, 8th edition, by G.J. Tortora, B.R. Funke
and C.L. Case, 2004
Microbiology: A Human Perspective by E.W. Nester, C.E. Roberts,
M.T. Nester, D.G. Anderson, 2005
Instructor prepared lab manual