Untitled document
At the conclusion of this course, the student should be able to:
1. Apply basic chemistry theory and lab practice in a wine lab.
2. Understand the set-up and accurate use of major lab instruments used in a medium-sized wine lab.
3. Understand the theory and procedures to assay major wine components analyzed in a medium-sized wine lab.
4. Understand the basic theory behind procedures normally performed in a state-of-the art wine lab, including genetic, microbiological, and chemical assays.
Untitled document
I. Basic Chemistry Theory and Practice
A. Lab safety
B. Wine lab analysis (chemical theory, formulas and equations)
C. Principles of enzymatic analyses of wine
D. Procedures for preparing wine samples
E. Disposal of samples and reagents
F. Lab data collection process and recordkeeping
G. Lab assay results and reports
H. Sanitation in lab areas
J. Standardize sodium hydroxide
II. Wine lab instruments
A. pH meter
B. Manual titratable acidity glassware
C. Autotitrator for titratable acidity
D. Centrifuge
E. Conductivity meter
F. Dissolved oxygen meter
G. Confined space atmosphere monitor
H. Aeration-oxidation apparatus
I. Cash still
J. Spectrophotometer (UV-VIS)
K. Alcolyzer
L. Deionized water unit system
M. OenoFoss
N. Chemwell (Astoria Pacific) discrete analyzer
O. Gas chromatograph
P. Nephelometer
Q. Phase contrast microscope
R. Brightfield microscope
III. Assay principles and procedures for wine properties
A. pH
B. Titratable acidity
C. Turbidity
D. Alcohol
E. Residual sugar
F. Volatile acidity
G. Malic acid
H. Assimilable nitrogen
I. Sulfides
J. Proteins
K. Potassium bitartrate
L. Dissolved oxygen
M. Phenolics (tannins and pigments)
N. Total and free SO2
O. Wine spoilage organism presence and by-products
P. Other assays appropriate to medium-sized wine lab
IV. Theory of state-of-the art wine analyses
A. Fourier Transform Infrared Spectroscopy (FTIR)
B. High Pressure Liquid Chromatography (HPLC)
C. Gas Chromatography-Mass Spectrometry (GC- MS)
D. Scorpion assay for detection of DNA
E. Hemocytometer
Untitled document
Lecture Related Assignments:
1. Weekly reading (5-20 pages)
2. Exams (2-3), including a final exam
Lab Related Assignments:
1. 2-3 lab reports (1-6 pages each)
2. Weekly lab protocol preparation (1-2 pages each)
3. Worksheets (8-12)
4. Troubleshoot lab instrument problems (1-4)
5. Perform lab assays for final exam
Writing: Assessment tools that demonstrate writing skill and/or require students to select, organize and explain ideas in writing. | Writing 10 - 40% |
Lab Reports, Lab protocol preparation, Worksheets | |
Problem solving: Assessment tools, other than exams, that demonstrate competence in computational or non-computational problem solving skills. | Problem Solving 5 - 10% |
Troubleshoot lab instrument problems | |
Skill Demonstrations: All skill-based and physical demonstrations used for assessment purposes including skill performance exams. | Skill Demonstrations 10 - 40% |
Lab assays for final exam | |
Exams: All forms of formal testing, other than skill performance exams. | Exams 15 - 35% |
Exams, including a final exam | |
Other: Includes any assessment tools that do not logically fit into the above categories. | Other Category 10 - 25% |
Lab participation | |
Untitled document
Wine Analysis and Production. Zoecklein, Bruce W. et. al. Springer. 2013
Instructor prepared materials