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Upon completion of this course, the student should be able to:
1) Identify and demonstrate common laboratory equipment found in small animal veterinary practices.
2) Demonstrate use of hematrocrit tubes and perform a Packed Cell Volume and Total Protein analysis.
3) Discuss sample collection tubes including their use and appropriate sample handling techniques.
4) Demonstrate a manual complete blood count (CBC) including leukocyte identification.
5) Demonstrate a fecal floatation and identify common ova that may parasitize canine and feline patients.
6) Demonstrate a skin scraping and identify common ectoparasites that may infest canine and feline patients.
7) Discuss appropriate fine needle aspirate collection and handling.
8) Demonstrate collection and cytologic evaluation of samples from canine aural cytology.
9) Discuss common ophthalmologic evaluations performed on canine and feline patients.
10) Discuss the technology and technique in common ELISA (Enzyme-Linked Immunosorbent Assay) Snap tests.
11) Demonstrate proper sample collection and culture plating techniques for bacterial cultures.
12) Discuss technology used to create radiographs and evaluate radiograph protocols.
13) Identify personal protective equipment used in radiology and evaluate radiograph protocols for radiation risks and safety.
14) Discuss technology used in ultrasound examinations.
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1) Laboratory equipment and use
a. Infection and safety
b. Microscopes
c. Refractometers
d. Centrifuges
e. Blood analyzers
f. Collection tubes and sample handling
g. Stains and slide handling
h. Syringes and needle handling
i. Disposal protocols
2) Blood testing
a. Vascular anatomy and sample collection
b. Hematocrit tubes and Packed Cell Volume
c. Common serum chemistries and electrolytes
d. In-house chemistry machines
e. Hormone and drug assays
f. Veterinary laboratories and shipment protocols
3) Complete blood counts
a. Blood smear techniques
b. Erythrocyte lineage and identification
c. Leukocyte lineages and identification
d. Platelet lineages and identification
e. Blood cell abnormalities
f. Automated CBC machines
4) Fecal sample evaluation
a. Endoparasites and parasite lifecycles
b. Fecal sample collection and handling
c. Fecal floatation protocols
d. Fecal smear and sedimentation protocols
e. Examination and identification of parasite ova
5) Urine sample evaluation
a. Cystocentesis
b. Other urine collection techniques including catheterization
c. Urine handling and visual evaluation
d. Urine reagent strips
e. Urine cytology including crystals and casts
f. Urine specific gravity
6) Skin testing
a. Ectoparasites
b. Skin scrapes
c. Dermal impression smears
d. Trichogram and tape preparations
e. Dermatophyte testing
7) Cytology
a. Fine needle aspirates
b. Punch biopsies
c. Impression smears
d. Slide preparation, staining, and sample handling
e. Joint, marrow and special sample protocols
8) Ear testing
a. Otoscope use and ear canal evaluation
b. Aural sample collection
c. Aural cytology
9) Eye testing
a. Restraint and handling for ophthalmic exam
b. Ocular imaging
c. Fluorescein stains
d. Shirmer tear testing
e. Intraocular pressure measurement
10) Serology
a. Immune system review
b. Antibody and antigen assays
c. ELISA tests
d. Common in house Snap tests
11) Microbiology
a. Sample handling and collection
b. Bacterial growth and growth media
c. Agar plate and incubation protocols
d. Bacterial identification and speciation testing
e. Antibiotic sensitivity testing
f. PCR (Polymerase chain reaction) and other new testing modalities
12) Radiology
a. Patient positioning and specialized restraint equipment
b. Radiograph terminology
c. Image production and radiation emission
d. Safety and personal protective equipment
e. Radiograph techniques and settings
f. Film handling and development
g. Digital imaging
h. Contrast materials and special studies
i. Radiograph documentation and legalities
j. Equipment maintenance
13) Ultrasound
a. Patient positioning and specialized restraint equipment
b. Ultrasound terminology and image production
c. Abdominal ultrasonography
d. Echocardiography
e. Other ultrasound studies
14) Other imaging modalities
a. MRI (Magnetic Resonance Imaging)
b. CT (Computerized Tomography)
c. Nuclear Medicine
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Laboratory Procedures for Veterinary Technicians 5th ed by Charles Hendrix and Margi Sirois, published by Mosby, 2007
Applied Pharmacology for Veterinary Technicians 4th ed by Boyce Wanamaker and Kathy Massey, published by Saunders/Elsevier, 2009
Essential Calculations for Veterinary Nurses and Technicians 2nd ed by Terry Lake and Nicola Green, published by Butterworth-Heinemann, 2008
Instructor prepared materials