Upon successful completion of this course, students will be able to:
1. Define microorganism and categorize microbes by domain and kingdom.
2. Describe the history of the discovery of the microbial world.
3. Relate microbial causality of disease to Koch's Postulates.
4. Describe the basic chemical activities essential to life.
5. Describe the structure of prokaryotic and eukaryotic cells.
6. Contrast genetic mutation, recombination, conjugation, transformation, transduction.
7. Describe viruses, their relation to cells, and vaccinations.
8. Compare various mechanisms of pathogenicity.
9. Describe the function of the immune system and its relation to disease.
10. Relate environmental influences on host resistance to public health measures.
11. Perform basic microbiological laboratory techniques.
1. History of microbiology
A. Discovery, microscopy, staining
B. Koch's Postulates and causality
C. Scientific method as it applies to microbiology
D. Microbiology and world civilizations
2. Unity of life
A. Cells and chemistry
B. Structure and function of nucleic acids
C. Structure and function of proteins
D. Energy metabolism
E. Prokaryotes and eukaryotes
F. Antibiotics and selective toxicity
3. Taxonomy and identification
A. DNA based methodologies
C. Select normal flora and pathogens
4. Microbial genetics
A. Mutation and recombination
1. plasmids, conjugation, transduction, transformation
B. Antibiotic paradox
A. Discovery and definitions
B. Interactions with host cell
C. Anti-viral vaccination and chemotherapy
D. Retrovirus, HIV disease, cancer
7. Host's role in disease
B. Non-specific resistance
C. The immune system and immunization
D. Environmental influences on host resistance
8. Lab exercises
A. Laboratory safety and sanitation
B. Laboratory techniques
1. aseptic techniques
2. bacterial culture (liquid and solid medium)
3. microscopy and staining techniques
4. preparation and sterilization of media
5. analyses of bacteria in water samples and on the human skin
6. antibiotic sensitivity
7. metabolic tests and bacterial identification
8. ELISA (enzyme-linked immunosorbent assay)
1. Reading assignments from text, averaging one chapter per week;
additional reading assignments averaging 5-10 pages per week
2. Research paper- involves library or internet research and 10 minute oral presentation
3. Examinations: objective and essay questions, 3 midterms, 2 lab practical exams, and a final
4. Laboratory experiments, data collection, demonstration of sterile and
Microbiology: An Introduction, 10th edition, G.J. Tortora, B.R. Funke
and C.L. Case, 2009
Microbiology: A Systems Approach, 2nd edition, M.K.Cowan and K.P. Talaro, McGraw-Hill, 2009
Instructor prepared lab manual